Figure 4.

Accumulation of gp27 in the ER–Golgi 15°C compartment. Vero cells were infected with VSV-G^ts045 and kept for 3 h at the restrictive temperature. After addition of the protein synthesis inhibitor cycloheximide, cells were further incubated for 1 h at 15°C. Peripheral structures of gp27 grew in number and apparent size. Note the high degree of coincident staining with VSV-G^ts045 glycoprotein arrested on its way from ER to the Golgi apparatus. Bar, 10 μm.