Figure 8.

Coexpression of p24 family proteins allows transport of gp27 from ER to the Golgi apparatus. HeLa cells were transiently transfected with gp27 cDNA (20%) and other p24 family cDNAs (20% each, pcDNA3 vector DNA making up to 100%). The Golgi apparatus was identified by mAb GTL2 against GalT. Without coexpressed p24 proteins, the predominant staining pattern for overexpressed gp27 is ER. Double transfection of gp27 with other p24 family cDNAs gives different phenotypes depending on which particular cDNA is used. In terms of Golgi localization, coexpression of p23, p24, and p25 is the most efficient combination. See table and text for details. The localization indicated in parentheses in the table is not the predominant phenotype but was observed consistently. Bar, 10 μm.