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Glycosylation analysis of gp27 and GMP25 in HeLa cells. (a) Removal of N-linked oligosaccharides by peptide N-glycanase F led to faster migration of both proteins, but p26 is not affected. Maturation of the oligosaccharide chain of gp27 in the medial-Golgi renders it resistant to digestion with Endo H. The amount of Endo H–sensitive GMP25 is ≥95%. (b) Pulse–chase analysis of the acquisition of Endo H resistance for gp27. After 60 min, two-thirds of gp27 are already Endo H resistant.
