Table 2.

Quantification of immunoprecipitated proteins using antibodies against gp27, p23, and p26

	gp27 Ip count	gp27 Ip (%)	p23 Ip count	p23 Ip (%)	p26 Ip count
gp27 (0,−1)	144,855	68	12,306	10
p23	50,811	9.5	99,580	53
p24	77,187	7.3	40,212	10
GMP25	64,629	15	41,088	26
p26					70,461
pX					43,022
Background	941		2,556		7,644

All immunoprecipitations (Ip) were done with the same lysate. Although the coprecipitation using gp27 antibodies in this case was less efficient for p23 and p24 compared with Figure 7C, counts were still high above background. In the area corresponding to p26, no signal above background was observed. Note that p23 also coprecipitates three other p24 family proteins: gp27, p24, and GMP25. Immunoprecipitation with p26 antibodies did not give any signal in the areas corresponding to gp27, p23, p24, or GMP25. Instead, a protein (pX) with an isoelectric point close to p26 was coprecipitated. Because the methionine content of this protein is unknown, no further calculation is possible. Blank spaces in the table represent no significant counts above background.