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. Author manuscript; available in PMC: 2008 Sep 17.
Published in final edited form as: Bioconjug Chem. 2005;16(5):1282–1290. doi: 10.1021/bc050201y

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Composition of E4 mixture as a function of conjugation methodology. HICHPLC chromatograms were integrated to determine the abundance of E0, E2, E4, E6, and E8 in the mixtures. Values are ± standard deviation for 4 (DTT partial reduction), 3 (TCEP partial reduction), or 6 (DTNB partial reoxidation) separate experiments. The contributions from odd-loaded species, typically less than 10%, are not shown. The sum of E0, E2, E4, E6, and E8 was normalized to 100%. The simulated reduction assumes that the rate constants for reduction of the 4 interchain disulfides are equal for a random reduction process, and the sum of E0, E2, E4, E6, and E8 equals 100%.