. Author manuscript; available in PMC: 2008 Sep 17.

Published in final edited form as: Bioconjug Chem. 2005;16(5):1282–1290. doi: 10.1021/bc050201y

Table 1.

Time course of isomer distribution for DTT partial reduction. Numbers given are in mole fraction. For each drug loading level, the sum of the isomers is equal to 100%.

Time (min)^a	Drugs/mAb^b	E2A^c	E2B^c	E4A^d	E4B^e	E4C^e	E6A^f	E6B^f
10	1.3	12	88	9	63	28	N/D	N/D
20	2.1	9	91	7	65	29	7	93
35	2.7	9	91	7	63	31	6	94
55	3.3	9	91	7	63	30	8	92
80	3.6	9	92	7	61	32	6	94
120	3.9	11	90	8	61	31	7	93

Reduction time. Once reduced, all antibodies were treated with vcMMAE for identical times.

Determined by HIC-HPLC.

Determined from reversed-phase HPLC data using Equation 1.

Determined from capillary electrophoresis data using Equation 3.

Determined from reversed-phase HPLC data using Equations 4 and 5.

Determined from reversed-phase HPLC data using Equation 2. N/D, not determined. At this time point, very little E6 was produced and this material was not sufficient for determining the isomer population.

Time (min)^a	Drugs/mAb^b	E2A^c	E2B^c	E4A^d	E4B^e	E4C^e	E6A^f	E6B^f
10	1.3	12	88	9	63	28	N/D	N/D
20	2.1	9	91	7	65	29	7	93
35	2.7	9	91	7	63	31	6	94
55	3.3	9	91	7	63	30	8	92
80	3.6	9	92	7	61	32	6	94
120	3.9	11	90	8	61	31	7	93

Time (min)^a	Drugs/mAb^b	E2A^c	E2B^c	E4A^d	E4B^e	E4C^e	E6A^f	E6B^f
10	1.3	12	88	9	63	28	N/D	N/D
20	2.1	9	91	7	65	29	7	93
35	2.7	9	91	7	63	31	6	94
55	3.3	9	91	7	63	30	8	92
80	3.6	9	92	7	61	32	6	94
120	3.9	11	90	8	61	31	7	93

Time (min)^a	Drugs/mAb^b	E2A^c	E2B^c	E4A^d	E4B^e	E4C^e	E6A^f	E6B^f
10	1.3	12	88	9	63	28	N/D	N/D
20	2.1	9	91	7	65	29	7	93
35	2.7	9	91	7	63	31	6	94
55	3.3	9	91	7	63	30	8	92
80	3.6	9	92	7	61	32	6	94
120	3.9	11	90	8	61	31	7	93