. Author manuscript; available in PMC: 2008 Sep 17.

Published in final edited form as: Bioconjug Chem. 2005;16(5):1282–1290. doi: 10.1021/bc050201y

Table 2.

In vitro binding and cytotoxicity of cAC10-vcMMAE.

ADC	Binding K_d (nM)^a	Karpas 299 IC₅₀ (ng/mL)^b
E0 (cAC10)	2.70 ± 1.91	N/D
E2 mixture DTT	N/D	11.4 ± 2.4
E2 DTT	3.57 ± 2.41	13.8 ± 3.6
E2 mixture DTNB	N/D	11.7 ± 4.5
E2 DTNB	2.02 ± 1.22	13.2 ± 2.7
E4 mixture DTT	N/D	3.4 ± 1.2
E4 DTT	7.76 ± 3.93	4.8 ± 0.7
E4 mixture DTNB	N/D	5.0 ± 0.0
E4 DTNB	7.69 ± 4.42	4.3 ± 0.9
E8	6.53 ± 3.09	2.7 ± 0.2

Binding to Karpas 299, determined from 4−7 independent measurement ± standard deviation. N/D, not determined.

In vitro cytotoxicity, in ng cAC10/mL, determined from 3 independent measurements ± standard deviation. N/D, not determined. cAC10 alone displays poor activity against Karpas 299.