Abstract
Reovirus guanylyltransferase, studied as a covalent enzyme-GMP intermediate, was used to guanylate appropriate acceptor molecules in vitro to produce authentic cap structures. Guanylyltransferase activity was associated with lambda 2, the 140-kilodalton product of the L2 gene segment of reovirus serotypes 1 and 3.
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- Borsa J., Graham A. F. Reovirus: RNA polymerase activity in purified virions. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895–901. doi: 10.1016/0006-291x(68)90396-3. [DOI] [PubMed] [Google Scholar]
- Borsa J., Grover J., Chapman J. D. Presence of nucleoside triphosphate phosphohydrolase activity in purified virions of reovirus. J Virol. 1970 Sep;6(3):295–302. doi: 10.1128/jvi.6.3.295-302.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carter C. A. Methylation of reovirus oligonucleotides in vivo and in vitro. Virology. 1977 Jul 15;80(2):249–259. doi: 10.1016/s0042-6822(77)80002-0. [DOI] [PubMed] [Google Scholar]
- Chang C. T., Zweerink H. J. Fate of parental reovirus in infected cell. Virology. 1971 Dec;46(3):544–555. doi: 10.1016/0042-6822(71)90058-4. [DOI] [PubMed] [Google Scholar]
- Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
- Drayna D., Fields B. N. Activation and characterization of the reovirus transcriptase: genetic analysis. J Virol. 1982 Jan;41(1):110–118. doi: 10.1128/jvi.41.1.110-118.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Faust M., Millward S. In vitro methylation of nascent reovirus mRNA by a virion-associated methyl transferase. Nucleic Acids Res. 1974 Dec;1(12):1739–1752. doi: 10.1093/nar/1.12.1739. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Furuichi Y., Morgan M., Muthukrishnan S., Shatkin A. J. Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-. Proc Natl Acad Sci U S A. 1975 Jan;72(1):362–366. doi: 10.1073/pnas.72.1.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Furuichi Y., Muthukrishnan S., Tomasz J., Shatkin A. J. Mechanism of formation of reovirus mRNA 5'-terminal blocked and methylated sequence, m7GpppGmpC. J Biol Chem. 1976 Aug 25;251(16):5043–5053. [PubMed] [Google Scholar]
- Itoh N., Mizumoto K., Kaziro Y. Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties. J Biol Chem. 1984 Nov 25;259(22):13930–13936. [PubMed] [Google Scholar]
- Kapuler A. M., Mendelsohn N., Klett H., Acs G. Four base-specific nucleoside 5'-triphosphatases in the subviral core of reovirus. Nature. 1970 Mar 28;225(5239):1209–1213. doi: 10.1038/2251209a0. [DOI] [PubMed] [Google Scholar]
- Keith J. M., Venkatesan S., Gershowitz A., Moss B. Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylyltransferase from wheat germ. Biochemistry. 1982 Jan 19;21(2):327–333. doi: 10.1021/bi00531a020. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- McCrae M. A., Joklik W. K. The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology. 1978 Sep;89(2):578–593. doi: 10.1016/0042-6822(78)90199-x. [DOI] [PubMed] [Google Scholar]
- Mizumoto K., Kaziro Y., Lipmann F. Reaction mechanism of mRNA guanylyltransferase from rat liver: isolation and characterization of a guanylyl-enzyme intermediate. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1693–1697. doi: 10.1073/pnas.79.6.1693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nichols J. L., Bellamy A. R., Joklik W. K. Identification of the nucleotide sequences of the oligonucleotides present in reovirions. Virology. 1972 Aug;49(2):562–572. doi: 10.1016/0042-6822(72)90507-7. [DOI] [PubMed] [Google Scholar]
- Nishikawa Y., Chambon P. Purification of mRNA guanylyltransferase from calf thymus. EMBO J. 1982;1(4):485–492. doi: 10.1002/j.1460-2075.1982.tb01195.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ramig R. F., Cross R. K., Fields B. N. Genome RNAs and polypeptides of reovirus serotypes 1, 2, and 3. J Virol. 1977 Jun;22(3):726–733. doi: 10.1128/jvi.22.3.726-733.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roth M. J., Hurwitz J. RNA capping by the vaccinia virus guanylyltransferase. Structure of enzyme-guanylate intermediate. J Biol Chem. 1984 Nov 10;259(21):13488–13494. [PubMed] [Google Scholar]
- Shatkin A. J. Methylated messenger RNA synthesis in vitro by purified reovirus. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3204–3207. doi: 10.1073/pnas.71.8.3204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shatkin A. J., Sipe J. D. RNA polymerase activity in purified reoviruses. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462–1469. doi: 10.1073/pnas.61.4.1462. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shuman S., Hurwitz J. Mechanism of mRNA capping by vaccinia virus guanylyltransferase: characterization of an enzyme--guanylate intermediate. Proc Natl Acad Sci U S A. 1981 Jan;78(1):187–191. doi: 10.1073/pnas.78.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shuman S. RNA capping by HeLa cell RNA guanylyltransferase. Characterization of a covalent protein-guanylate intermediate. J Biol Chem. 1982 Jun 25;257(12):7237–7245. [PubMed] [Google Scholar]
- Skup D., Millward S. Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells. Nucleic Acids Res. 1977 Oct;4(10):3581–3587. doi: 10.1093/nar/4.10.3581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith R. E., Furuichi Y. A unique class of compound, guanosine-nucleoside tetraphosphate G(5')pppp(5')N, synthesized during the in vitro transcription of cytoplasmic polyhedrosis virus of Bombyx mori. Structural determination and mechanism of formation. J Biol Chem. 1982 Jan 10;257(1):485–494. [PubMed] [Google Scholar]
- Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
- Venkatesan S., Moss B. Eukaryotic mRNA capping enzyme-guanylate covalent intermediate. Proc Natl Acad Sci U S A. 1982 Jan;79(2):340–344. doi: 10.1073/pnas.79.2.340. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang D., Furuichi Y., Shatkin A. J. Covalent guanylyl intermediate formed by HeLa cell mRNA capping enzyme. Mol Cell Biol. 1982 Aug;2(8):993–1001. doi: 10.1128/mcb.2.8.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang D., Shatkin A. J. Synthesis of Gp4N and Gp3N compounds by guanylyltransferase purified from yeast. Nucleic Acids Res. 1984 Mar 12;12(5):2303–2315. doi: 10.1093/nar/12.5.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White C. K., Zweerink H. J. Studies on the structure of reovirus cores: selective removal of polypeptide lambda 2. Virology. 1976 Mar;70(1):171–180. doi: 10.1016/0042-6822(76)90247-6. [DOI] [PubMed] [Google Scholar]
- Yagi Y., Mizumoto K., Kaziro Y. Limited tryptic digestion of messenger RNA capping enzyme from Artemia salina. Isolation of domains for guanylyltransferase and RNA 5'-triphosphatase. J Biol Chem. 1984 Apr 25;259(8):4695–4698. [PubMed] [Google Scholar]
- Yamakawa M., Furuichi Y., Shatkin A. J. Priming of reovirus transcription by GppppG and formation of CpG(5')GpC. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6142–6146. doi: 10.1073/pnas.79.20.6142. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zarbl H., Hastings K. E., Millward S. Reovirus core particles synthesize capped oligonucleotides as a result of abortive transcription. Arch Biochem Biophys. 1980 Jul;202(2):348–360. doi: 10.1016/0003-9861(80)90437-3. [DOI] [PubMed] [Google Scholar]
- Zweerink H. J., Joklik W. K. Studies on the intracellular synthesis of reovirus-specified proteins. Virology. 1970 Jul;41(3):501–518. doi: 10.1016/0042-6822(70)90171-6. [DOI] [PubMed] [Google Scholar]