Figure 4.

VAMP4 and syntaxin 6 both localize to the same compartment upon BFA treatment. CHO cells, treated for 15 min with 10 μg/ml BFA, were fixed with 4% paraformaldehyde, permeabilized with saponin, and costained using affinity-purified rabbit anti-VAMP4 antibodies (A, D, and G) and mouse mAbs against syntaxin 6 (B), p115 (E), and transferrin receptor (H). Texas Red–labeled anti-rabbit IgG and FITC-labeled anti-mouse IgG were used as secondary antibodies before processing for indirect immunofluorescence microscopy. In yellow are areas of overlap in merged images (C, F, and I). Bar, 20 μm.