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Interaction of the PDZ domain of Enigma requires the carboxyl terminus of skeletal β-TM. Truncation mutants having deletions of 5 or 10 amino acids from the carboxyl terminus of skeletal β-TM (TMc5 and TMc10, respectively) were translated in vitro as T10-TM fusion proteins and incubated with GST-PDZEng or GST alone. Mutant skeletal β-TM proteins did not bind to GST-PDZEng. Input represents 2% of translation reactions of skeletal β-TM, TMc5, and TMc10 incubated with GST beads.
