Expression of Enigma in skeletal muscle, brain, and other tissues. (A) In situ hybridization of an E13.5 mouse embryo sagittal section shows Enigma mRNA expression in skeletal muscle. The arrow indicates Enigma expression within the intrinsic and extrinsic muscle mass of the tongue. (B) Expression is observed in epaxial, intercostal, and other skeletal muscles at the brachial level, including the latissimus dorsi muscle, indicated by the large arrow. Lens tissue is indicated by an arrowhead. (C) Higher magnification of lens tissue shown in B. The arrow marks the location of Enigma-expressing lens cells adjacent to the equatorial zone of proliferation. For orientation, anterior (A) and dorsal (D) axes are indicated in the inset. (D) Enigma mRNA is evident within the adult hippocampus, as indicated by the arrow.