Figure 7.

Localization of Enigma in skeletal muscle. (A) Immunofluorescence of semithin cryostat sections shows that Enigma is present at the Z line and some transverse filaments in adult muscle sarcomeres. Enigma is present along the Z line as indicated by the arrow. (B) Phalloidin-FITC staining of the same tissue section as in A shows the distribution of actin at the I band and at the Z line, which lies at the midline of the I band. The arrow marks the actin-rich Z line. (C) Digitally merging of the images of A and B shows codistribution of Enigma (red) and Phalloidin-stained actin (green). Regions where Enigma and actin overlap appear yellow, as indicated by the arrow. Arrows in A–C mark the same location in the tissue section. (D) Enigma is detected at the Z line, as indicated by the arrow, and along a subset of transverse filaments. (E) Skeletal β-TM is also distributed near the Z line (arrowhead) in a doublet pattern on the same tissue section as in D. Bar, ∼3 μM.