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Evidence for α-SNAP–α-SNAP interactions within 20S complexes. Standard cross-linking reactions were performed using coated vesicle-derived membranes without additions (lane 1), or with 1.5 μg (lane 2) or 50 μg (lane 3) GST, or 1.5 μg (lanes 4 and 5) GST-α-SNAP. Pellets from 20S complexes (lanes 1–4) or a secondary immunoprecipitation with anti-GST (lane 5) were analyzed for cross-linking products.
