Figure 1.

DNA and amino acid sequence comparison between the novel VAMP isoform VAMP-1B and VAMP-1A. (A) Comparison of cDNA sequence of clones encoding VAMP-1B and VAMP-1A (deduced from the genomic sequence). Solid arrows indicate limits of the coding sequences, and the open arrow indicates a splice junction at the 3′-end of the coding sequence. The VAMP-1B cDNA sequence has been deposited in GenBank with acccession number AF060538. (B) Carboxy-terminal hydrophobic amino acid sequences of VAMP-1A and human and rat VAMP-1B predicted from the corresponding cDNAs. Also shown are the sequences of BCL-2, monoamine oxidases A and B, and metaxin, four proteins anchored in mitochondria membranes by carboxy-terminal hydrophobic sequences. The hydrophobic sequences are underlined, and charged amino acids are indicated.