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MMP-2 expression and activity in Mpv17 transfectants. (A) Northern analysis of Mpv17-negative LUSVX cells and two different clones of LUSVX cells, stably transfected with different Mpv17 expression constructs (LuMpv9, LuMpv15). The two Mpv17 transcripts can be detected at 3.5 kb and 4.5 kb, respectively; 2.7 kb is the size of the MMP-2 transcript and 1.4 kb is the size of the GAPDH transcript. (B) Substrate gel analysis (Zymogram) of LUSVX, LU-Mpv9, and LU-Mpv15 cells.
