Figure 1.

Poly A⁺ RNA is bound by a ∼160-kDa protein in vivo. NGF-treated PC12 cells were preincubated with ³H-uridine and exposed to UV light. The cross-linked poly-A⁺ RNA–protein complexes were isolated by oligo-dT cellulose selection. The eluted radiolabeled protein species were resolved on a 10% SDS polyacrylamide gel and visualized by fluorography. The arrow denotes the ∼160-kDa RNA-binding protein.