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. 1999 Jun;10(6):2033–2050. doi: 10.1091/mbc.10.6.2033

Figure 2.

Figure 2

Expression of Cx43-GFP fusion protein in Cx43-deficient MDCK cells and communication-deficient HeLa cells. (I) Lysates of MDCK cells stably expressing Cx43-GFP were resolved by SDS-PAGE, and Western blots were immunolabeled with a polyclonal antibody to GFP (lane a), a polyclonal antibody directed against the amino terminus of Cx43 (lane b), or a monoclonal antibody directed against the carboxyl terminus of Cx43 (lane c). The asterisks denote the position of the major species of the Cx43-GFP fusion protein. (II) Recombinant GFP (lane a), lysates of HeLa cells (lane b), lysates of HeLa cells transiently expressing GFP (lane c), and lysates of HeLa cells transiently expressing Cx43-GFP (lane d) were probed with a polyclonal antibody to GFP and a monoclonal to Cx43 in an immunoblot.