Table 1.
Neurobiotin and Lucifer yellow transfer
| Cells | Transfection | Injected molecule | No. of injections | % coupled |
|---|---|---|---|---|
| NRK | Wild type | Neurobiotin | 55 | 100 |
| NRK + GFP | Transient | Neurobiotin | 50 | 88 |
| NRK + Cx43-GFP | Transient | Neurobiotin | 26 | 100 |
| HeLa | Wild type | Neurobiotin | 55 | 14 |
| HeLa + GFP | Transient | Neurobiotin | 47 | 15 |
| HeLa + Cx43-GFP | Transient | Neurobiotin | 74 | 86 |
| HeLa | Wild type | LY | 202 | 23 |
| HeLa + Cx43-GFP | Stable | LY | 217 | 78 |
| N2A | Wild type | LY | 73 | 4 |
| N2A + Cx43-GFP | Stable | LY | 48 | 93 |
In functional assays, wild type NRK, HeLa, and N2A cells and cells expressing Cx43-GFP or GFP were microinjected with Neurobiotin or Lucifer yellow (LY) to determine the incidence of gap junction coupling. The number of microinjected cells that resulted in Neurobiotin or LY transfer is expressed as a percent.