Figure 2.

Immunoprecipitation of Delta protein from detergent-soluble native extracts of staged embryos and larvae using an antibody against the Delta extracellular domain (MAb8A), detected with Delta MAb9B. (A) Staged embryonic extracts (6 mg wet weight/ml) immunoprecipitated using a mAb against the Delta extracellular domain (MAb8A). The same volume of extract was used for immunoprecipitation for each time point. Ages of pooled animals, in hours postoviposition (PO) at 25°C, for each lane are: lane 1, 0–6 h; lane 2, 7–12 h; lane 3, 13–18 h; lane 4, 19–24 h. The bracket next to “I1” indicates that more than one band is detected in the 92- to 96-kDa range. “HC” indicates the IgG heavy chain from the mouse ascites used in the immunoprecipitation. (B) Immunoprecipitation of Delta protein from third instar larval extracts (180 mg wet weight/ml) using MAb8A.