Figure 1.

Overexpression of Sec10 deletion mutants inhibits cell growth. (A) Hydropathy analysis and schematic representation of deletion mutants of Sec10p. The upper panel shows the regions in Sec10p that are homologous to C. elegans and human sequences and the region predicted to form a coiled-coil structure. The middle panel shows the hydropathy profile of Sec10p generated by Protean software (DNA star) based on the algorithm of Kyte and Doolittle (1982). Hydrophilic regions are represented by positive values and hydrophobic regions by negative values. The lower panels depict the deletion mutants of Sec10p. Numbers next to the cartoons indicate amino acids. (B) Inhibition of cell growth by overexpression of the Sec10p domains, Sec10ΔC and Sec10CT. Wild-type yeast or yeast expressing the Sec10 mutants (Sec10ΔC [ΔC], Sec10 CT [CT], and Sec10NT [NT]) from the GAL1 promoter were grown at 25°C on rich medium containing glucose (YPD), a repressing carbon source, or galactose (YPGal) for the induction of the mutants. The order of strains is depicted schematically on the right.