Figure 2.

Phenotypic analysis of cells expressing the Sec10p dominant-negative mutants. Wild-type yeast (A, D, G, and J) or yeast expressing the dominant-negative Sec10p mutants Sec10ΔC (B, E, H, and K), and Sec10CT (C, F, I, and L) after a 10-h induction in galactose were fixed and processed for immunofluorescence. Double labeling was performed with polyclonal anti-Sec4p antibodies (D–F) followed by monoclonal anti-actin antibodies (G–I), and DAPI staining (J–L). The morphology of the cells in the same field was observed under phase-contrast with differential interference (DIF; A–C).