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Sec10ΔC interacts with Sec15p in vitro. c-myc–tagged Sec10ΔC and members of the exocyst were cotranslated in vitro and immunoprecipitated with anti–c-myc antibodies. Two microliters of the translation reaction mixtures were loaded onto the gel (TnT) as a reference for the proteins present in the immunoprecipitation (IP).
