Figure 6.

Colocalization of Sec15p and Sec4p upon Sec15p overexpression. Wild-type yeast (A and B) or yeast overexpressing Sec15p (C and D) or cooverexpressing Sec15p with Sec10ΔC (E and F) or with Sec10CT (G and H) were grown in galactose for 10 h to induce overexpression of the proteins. Cells were then processed for immunofluorescence as described in MATERIALS AND METHODS. Double labeling was performed with monoclonal anti-Sec4p antibodies (A, C, E, and G) and polyclonal anti-Sec15p antibodies (B, D, F, and H).