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Myo2p colocalizes with Sec4p. Yeast were grown for 10 h in galactose to induce the overexpression of Sec10ΔC, Sec10CT, and Sec15p. Cells were then processed for immunofluorescence and costaining performed with anti-Myo2p (A, C, E, and G) and anti-Sec4p antibodies (B, D, F, and H). (A and B) Wild-type. (C and D) Cells overexpressing Sec15p. (E and F) Cells co-overexpressing Sec15p with Sec10ΔC. (G and H) Cells co-overexpressing Sec15p with Sec10CT.
