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. 1987 May;61(5):1615–1620. doi: 10.1128/jvi.61.5.1615-1620.1987

Replicative cis-advantage of polyomavirus regulatory region mutants in different murine cell lines.

V De Simone, P Amati
PMCID: PMC254143  PMID: 3033287

Abstract

To determine the relative growth advantages of polyomavirus regulatory region mutants selected from Friend leukemic and neuroblastoma cells persistently infected with polyomavirus A2 wild type, different murine cell lines, some of which are capable of further differentiation in vitro, were used as hosts in mixed infection and transfection. The tests allowed the determination, through the measurement of cis-advantage in replication, of the most effective polyomavirus regulatory region constitutions for a given cell line and, in some cases, for specific stages of cell differentiation. Furthermore, different domains of the viral regulatory region were shown to have different effects--positive, neutral, or negative--depending on the host analyzed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amati P. Polyoma regulatory region: a potential probe for mouse cell differentiation. Cell. 1985 Dec;43(3 Pt 2):561–562. doi: 10.1016/0092-8674(85)90225-9. [DOI] [PubMed] [Google Scholar]
  2. Boccara M., Kelly F. Expression of polyoma virus in heterokaryons between embryonal carcinoma cells and differentiated cells. Virology. 1978 Oct 1;90(1):147–150. doi: 10.1016/0042-6822(78)90342-2. [DOI] [PubMed] [Google Scholar]
  3. Cowie A., Kamen R. Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyomavirus DNA. J Virol. 1984 Dec;52(3):750–760. doi: 10.1128/jvi.52.3.750-760.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dailey L., Basilico C. Sequences in the polyomavirus DNA regulatory region involved in viral DNA replication and early gene expression. J Virol. 1985 Jun;54(3):739–749. doi: 10.1128/jvi.54.3.739-749.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Simone V., La Mantia G., Lania L., Amati P. Polyomavirus mutation that confers a cell-specific cis advantage for viral DNA replication. Mol Cell Biol. 1985 Aug;5(8):2142–2146. doi: 10.1128/mcb.5.8.2142. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Delli Bovi P., De Simone V., Giordano R., Amati P. Polyomavirus growth and persistence in Friend erythroleukemic cells. J Virol. 1984 Feb;49(2):566–571. doi: 10.1128/jvi.49.2.566-571.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujimura F. K., Linney E. Polyoma mutants that productively infect F9 embryonal carcinoma cells do not rescue wild-type polyoma in F9 cells. Proc Natl Acad Sci U S A. 1982 Mar;79(5):1479–1483. doi: 10.1073/pnas.79.5.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Herbomel P., Bourachot B., Yaniv M. Two distinct enhancers with different cell specificities coexist in the regulatory region of polyoma. Cell. 1984 Dec;39(3 Pt 2):653–662. doi: 10.1016/0092-8674(84)90472-0. [DOI] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Katinka M., Yaniv M. DNA replication origin of polyoma virus: early proximal boundary. J Virol. 1983 Jul;47(1):244–248. doi: 10.1128/jvi.47.1.244-248.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katinka M., Yaniv M., Vasseur M., Blangy D. Expression of polyoma early functions in mouse embryonal carcinoma cells depends on sequence rearrangements in the beginning of the late region. Cell. 1980 Jun;20(2):393–399. doi: 10.1016/0092-8674(80)90625-x. [DOI] [PubMed] [Google Scholar]
  12. Kern F. G., Dailey L., Basilico C. Common regulatory elements control gene expression from polyoma early and late promoters in cells transformed by chimeric plasmids. Mol Cell Biol. 1985 Aug;5(8):2070–2079. doi: 10.1128/mcb.5.8.2070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maione R., Passananti C., De Simone V., Delli-Bovi P., Augusti-Tocco G., Amati P. Selection of mouse neuroblastoma cell-specific polyoma virus mutants with stage differentiative advantages of replication. EMBO J. 1985 Dec 1;4(12):3215–3221. doi: 10.1002/j.1460-2075.1985.tb04068.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Melin F., Pinon H., Reiss C., Kress C., Montreau N., Blangy D. Common features of polyomavirus mutants selected on PCC4 embryonal carcinoma cells. EMBO J. 1985 Jul;4(7):1799–1803. doi: 10.1002/j.1460-2075.1985.tb03853.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Muller W. J., Mueller C. R., Mes A. M., Hassell J. A. Polyomavirus origin for DNA replication comprises multiple genetic elements. J Virol. 1983 Sep;47(3):586–599. doi: 10.1128/jvi.47.3.586-599.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Norkin L. C. Papovaviral persistent infections. Microbiol Rev. 1982 Dec;46(4):384–425. doi: 10.1128/mr.46.4.384-425.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ruley H. E., Fried M. Sequence repeats in a polyoma virus DNA region important for gene expression. J Virol. 1983 Jul;47(1):233–237. doi: 10.1128/jvi.47.1.233-237.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Tyndall C., La Mantia G., Thacker C. M., Favaloro J., Kamen R. A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication. Nucleic Acids Res. 1981 Dec 11;9(23):6231–6250. doi: 10.1093/nar/9.23.6231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Veldman G. M., Lupton S., Kamen R. Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression. Mol Cell Biol. 1985 Apr;5(4):649–658. doi: 10.1128/mcb.5.4.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  22. Weber F., de Villiers J., Schaffner W. An SV40 "enhancer trap" incorporates exogenous enhancers or generates enhancers from its own sequences. Cell. 1984 Apr;36(4):983–992. doi: 10.1016/0092-8674(84)90048-5. [DOI] [PubMed] [Google Scholar]
  23. Wigler M., Silverstein S., Lee L. S., Pellicer A., Cheng Y. c., Axel R. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells. Cell. 1977 May;11(1):223–232. doi: 10.1016/0092-8674(77)90333-6. [DOI] [PubMed] [Google Scholar]
  24. de Villiers J., Schaffner W., Tyndall C., Lupton S., Kamen R. Polyoma virus DNA replication requires an enhancer. Nature. 1984 Nov 15;312(5991):242–246. doi: 10.1038/312242a0. [DOI] [PubMed] [Google Scholar]

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