Table 2.
Loci | Alleles | Morphologya | Microtubules | Genes/product |
---|---|---|---|---|
alp 1 | 1315, 1356, 1584 | Bent/branched | No | Cofactor Db |
2 | 996, 1212, 1377 | Bent/branched | No | atb2+/α2-tubulin |
3 | 149, 601 | Curved/cut | ||
4 | 225, 1891 | Bent/cut | Few | |
5 | 1134 | Bent | ||
6 | 719 | Bent/cut | ||
7 | 738 | Bent/cut | ||
8 | 1146c | Bent/branched | tea1+ | |
9 | 1437 | Bent | ||
10 | 103 | Curved | ||
11 | 924 | Bent/branched | No | Cofactor Bd |
12 | 1828 | Bent/branched | No | nda3+/β-tubulin |
13 | 1837 | Bent/cut | ||
14 | 1270 | Bent/branched | Few | |
15 | 1218 | Bent/cut |
Mutants that showed >1% of cells with branched morphology or 10% of “cut” at 36°C for 8 h are indicated as “branched” or “cut” (Hirano et al., 1986), respectively.
The original isolate (1146) contained two mutations; one in tea1 (Mata and Nurse, 1997), which leads to branched morphology at 36°C, and the other in an unknown locus, which causes temperature-sensitive growth.
Radcliffe and Toda, unpublished observations.