Table 1.
Localization of AMF-R in HeLa and NIH-3T3 cells by immunoelectron microscopy
| Smooth tubules and vesicles | Rough ER | Flat plasma membrane | Caveolae | Clathrin-coated pits and vesicles | |
|---|---|---|---|---|---|
| HeLa | |||||
| AMF-R | |||||
| Gold particles (n) | 660 | 34 | 147 | 52 | 3 |
| Membrane (μm) | 328.5 | 187.5 | 245.6 | 14.6 | 19.3 |
| Gold particles/μm | 2.01 ± 0.15 | 0.18 ± 0.04 | 0.60 ± 0.08 | 3.56 ± 0.53 | 0.16 ± 0.13 |
| Control | |||||
| Gold particles (n) | 83 | 6 | 25 | 3 | 1 |
| Membrane (μm) | 307.5 | 95.2 | 211.0 | 16.1 | 5.0 |
| Gold particles/μm | 0.27 ± 0.08 | 0.06 ± 0.03 | 0.12 ± 0.05 | 0.19 ± 0.10 | 0.20 ± 0.21 |
| NIH-3T3 | |||||
| AMF-R | |||||
| Gold particles (n) | 640 | 74 | 296 | 44 | 2 |
| Membrane (μm) | 432.6 | 308.9 | 308.8 | 28.8 | 34.7 |
| Gold particles/μm | 1.48 ± 0.10 | 0.24 ± 0.06 | 0.96 ± 0.10 | 1.53 ± 0.30 | 0.06 ± 0.06 |
| Control | |||||
| Gold particles (n) | 33 | 7 | 10 | 4 | 2 |
| Membrane (μm) | 303.1 | 109.8 | 138.1 | 31.7 | 6.2 |
| Gold particles/μm | 0.11 ± 0.02 | 0.06 ± 0.03 | 0.07 ± 0.03 | 0.13 ± 0.06 | 0.32 ± 0.18 |
Gold particles associated with the indicated membrane organelles were counted and the density per μm membrane length determined. Control labeling was determined using a nonimmune rat IgM antibody (Benlimame et al., 1995).