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. 1987 May;61(5):1712–1716. doi: 10.1128/jvi.61.5.1712-1716.1987

Expression of polyomavirus large T antigen by using a baculovirus vector.

W C Rice, H E Lorimer, C Prives, L K Miller
PMCID: PMC254162  PMID: 3033300

Abstract

A gene encoding the large T antigen of polyomavirus was inserted into the baculovirus Autographa californica nuclear polyhedrosis virus so that gene expression was under the control of the strong, very late polyhedrin gene promoter. Significantly more large T antigen was produced in recombinant virus-infected insect cells than was observed in polyomavirus-transformed mouse cells. The insect-derived T antigen exhibited polyomavirus origin-specific DNA binding. The baculovirus expression system provides a convenient source of T antigen for in vitro studies.

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Selected References

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