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. 1999 Jun;10(6):2075–2086. doi: 10.1091/mbc.10.6.2075

Figure 3.

Figure 3

The utrophin N-Box binds proteins in nuclear extracts of cultured L6 myotubes. EMSA was performed with the radiolabeled UtroNBox probe using 10 μg of nuclear extract made from cultured L6 myotubes. Lane 1, migration of free, unretarded probe control; lane 2, mobility shift of the probe in the presence of a N-box–binding factor present in L6 nuclear extracts; lane 3, control for specificity showing that the mobility shift was competed with a 1000× excess of unlabeled probe.