Basolateral localization of LET-23 requires lin-10. Immunofluorescent micrographs of vulval precursor cells stained with anti-LET-23 antibodies (green) and a cell junction mAb marker (MH27, red) in lin-10(sy217) (A–D) and lin-10(n1509) (E) mutants are shown. (A) Lateral view of an early L2 hermaphrodite. (B) Lateral view of a late L2–early L3 hermaphrodite. Optical cross-sections of P6.p (C) and P8.p (D) in a late L2–early L3 hermaphrodite were generated as in Figure 1, D and E. (E) Lateral view of the two daughter cells from P6.p. Bars, 10 μm (A, B, and E); 2 μm (C and D).