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. 1999 Jun;10(6):2087–2100. doi: 10.1091/mbc.10.6.2087

Figure 5.

Figure 5

LIN-10 is truncated in lin-10(n1853) and absent in two other mutants. LIN-10 was detected by analyzing protein extracts from hermaphrodites using Western blots and anti-LIN-10 antibodies. Full-length LIN-10 is indicated by an arrow. A smaller LIN-10 product (*) may correspond to a LIN-10 degradation product or an alternate LIN-10 isoform. Truncated LIN-10 product in lin-10(n1853) is indicated by an arrowhead. The lin-10(n1853) truncation does not affect the epitope used to generate anti-LIN-10 antibodies (see MATERIALS AND METHODS). No LIN-10 expression is observed in a deletion mutant, lin-10(n299), or in a mutant carrying an early stop mutation, lin-10(sy217). Molecular size markers are shown.