Figure 10.

A role for the LIM domains of paxillin in regulating fibroblast adhesion to fibronectin. Blocking phosphorylation of T403 or S481 reduces cell adhesion by 25% whereas mimicking constitutive phosphorylation of these residues stimulates adhesion by 25%. (A) LIM2^T403V and LIM2^T403E. (B) LIM3^S457A and LIM3^S457D. (C) LIM3^S481A and LIM3^S481D. Avian paxillin transfectants were maintained in suspension for 1 h before adhesion for 30 min on 10 μg/ml fibronectin-coated, 1% BSA-blocked 96-well dishes, 8 wells per transfectant. After extensive washing, absorbance values were obtained by MTT assay and adhesion relative to wild-type avian paxillin-expressing transfectants was calculated. Each graph represents the mean and SD from the mean from three adhesion assays, performed in duplicate. Statistical analyses were performed with Student’s t test; *, p < 0.05; **, p < 0.01.