Figure 9.

Adhesion of CHO.K1 cells expressing avian paxillin containing mutations of Y31/118F and S188/190A is identical to cells expressing wild-type avian paxillin. Avian paxillin transfectants were maintained in suspension for 1 h before adhesion for 30 min on 10 μg/ml fibronectin-coated, 1% BSA-blocked 96-well dishes, 8 wells per transfectant. After extensive washing, absorbance values were obtained by MTT assay, and adhesion relative to wild-type avian paxillin-expressing transfectants was calculated. Four independent experiments were executed, in duplicate, and tabulated to determine mean and SD from the mean. Statistical analyses were performed with Student’s t test; *, p < 0.05; **, p < 0.01.