Abstract
Preparation of monoclonal antibodies to bovine virus diarrhea virus (BVDV) yielded some hybridoma cells that secreted monoclonal antibodies against the Madin-Darby bovine kidney cells. The anti-cellular monoclonal antibodies reacted with other bovine cells (bovine turbinate and testicle) but not with cell lines derived from other animal species. Subclones derived from one hybridoma partially blocked the infectivity of BVDV, possibly through the binding of the monoclonal antibodies with an epitope close to the receptor site of BVDV and not by way of steric hindrance. Unexpectedly, these same subclones completely blocked the infectivity of bovine enterovirus-3 (BEV-3) strain 240A and partially blocked the infectivity of BEV-2 and BEV-3 (ATCC strain) but not that of other serotypes. Other subclones derived from two other hybridomas, although cell membrane specific, did not have a protective activity against BEV or BVDV.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Campbell B. A., Cords C. E. Monoclonal antibodies that inhibit attachment of group B coxsackieviruses. J Virol. 1983 Nov;48(2):561–564. doi: 10.1128/jvi.48.2.561-564.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colonno R. J., Callahan P. L., Long W. J. Isolation of a monoclonal antibody that blocks attachment of the major group of human rhinoviruses. J Virol. 1986 Jan;57(1):7–12. doi: 10.1128/jvi.57.1.7-12.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Crowell R. L., Field A. K., Schleif W. A., Long W. L., Colonno R. J., Mapoles J. E., Emini E. A. Monoclonal antibody that inhibits infection of HeLa and rhabdomyosarcoma cells by selected enteroviruses through receptor blockade. J Virol. 1986 Feb;57(2):438–445. doi: 10.1128/jvi.57.2.438-445.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunne H. W., Huang C. M., Lin W. J. Bovine enteroviruses in the calf: an attempt at serologic, biologic, and pathologic classification. J Am Vet Med Assoc. 1974 Feb 1;164(3):290–294. [PubMed] [Google Scholar]
- Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
- Lecomte J. Hybridoma antibodies produced against bromelain derived cores of influenza virus. Microbiol Immunol. 1985;29(3):251–263. doi: 10.1111/j.1348-0421.1985.tb00824.x. [DOI] [PubMed] [Google Scholar]
- Minor P. D., Pipkin P. A., Hockley D., Schild G. C., Almond J. W. Monoclonal antibodies which block cellular receptors of poliovirus. Virus Res. 1984;1(3):203–212. doi: 10.1016/0168-1702(84)90039-x. [DOI] [PubMed] [Google Scholar]
- Nobis P., Zibirre R., Meyer G., Kühne J., Warnecke G., Koch G. Production of a monoclonal antibody against an epitope on HeLa cells that is the functional poliovirus binding site. J Gen Virol. 1985 Dec;66(Pt 12):2563–2569. doi: 10.1099/0022-1317-66-12-2563. [DOI] [PubMed] [Google Scholar]
- Schiff L. J., Storz J. Kinetics of virus production in cells doubly infected with the virus of bovine diarrhea and a bovine enterovirus. Arch Gesamte Virusforsch. 1972;36(3):218–225. doi: 10.1007/BF01249852. [DOI] [PubMed] [Google Scholar]
- Tomassini J. E., Colonno R. J. Isolation of a receptor protein involved in attachment of human rhinoviruses. J Virol. 1986 May;58(2):290–295. doi: 10.1128/jvi.58.2.290-295.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]