Table 1.
Levels of expression in independently derived HSC3 and HSC4 fly lines
| Transgenic fly linea | Levels of expressionb |
|---|---|
| 4/WT/A | + |
| 4/WT/B | ++ |
| 4/206/D | + |
| 4/206/E | ++ |
| 4/71/I | + |
| 4/71/G | ++ |
| 3/WT/A | + |
| 3/WT/B | ++ |
| 3/231/A | + |
| 3/231/D | +++ |
| 3/97/B | + |
| 3/97/D | +++ |
a
4/WT, HSC4 wild-type gene; 4/206, HSC4 with D206 substitution; 4/71, HSC4 with K71S substitution; 3/WT, HSC3 wild-type gene; 3/231, HSC3 with D231 substitution; 3/97, HSC3 with K97 substitution. Amino acid substitutions in HSC4(D206S, K71S) correspond to those substitutions in HSC3(D231S, K97S).
b
Fly strains producing the highest and lowest levels of accumulated mutant or wild-type proteins where +++ is ∼75–85% the level of wild-type proteins, ++ is ∼35–55% the level of wild-type protein, and + is ∼20–30% the level of wild-type protein.