Figure 2.

Fission yeast MCM protein complexes break down during gel filtration in the absence of glycerol. Wild-type cell lysate was fractionated over a Superose 6 gel filtration column, using lysis buffer as the elution buffer. Samples from each fraction were separated by SDS-PAGE, and duplicate filters were immunoblotted with antibodies to fission yeast MCM proteins, as indicated. The asterisk (*) shows a protein that cross-reacts with the Mis5p antibody (see Figure 1B). Gel filtration markers, bovine thyroglobulin (670 kDa) and bovine γ-globulin (158 kDa), are indicated by the vertical arrows.