Table 1.

Summary of Cdc19 mutants^a

Mutant	Mutation	Region affected	Comp.b	MCM binding
wt			+	++
D1	Δ50-79	Acidic region	+	++
D2	Δ80-104	Acidic region	+	++
D3	Δ137-143	Acidic region	+	++
D4	Δ45-126	Acidic region	−	−
D6	Δ736-830	C terminus	−	−
D7	Δ1-204	N terminus	−	−
D8	Δ528-613	MCM homology core	−	−
D9	Δ615-724	MCM homology core	−	−
D10	Δ528-724	MCM homology core	−	−
M1	C334S	Putative zinc finger	−	−
M2	C337S	Putative zinc finger	−	−
M3	C357S	Putative zinc finger	−	−
M4	C360S	Putative zinc finger	−c	−
M5	G534R	Putative NTP binding site	−	−
M6	K540A	Putative NTP binding site	−	+
M7	K540R	Putative NTP binding site	+	+
M8	D598A	Putative NTP binding site	−	+
M9	R7E R9D	Putative NLS1d	−	++
M10	A113T R116E R117E	Putative NLS2d	−	++
P1 (ts)	P257L T272I		−	−

^a Mutants were described by Forsburg et al. (1997). 

^b Complementation of the cdc19-P1 mutant at 36°C. Data from Forsburg et al. (1997). 

^c Our unpublished data. 

^d NLS, nuclear localization sequence.