Table 2.
qRT-PCR confirmation of differential expression of selected transcriptsa
| 4S vs. 2AS (fold) | 9S vs. 3AS (fold) | |||
| Gene Symbol | qRT-PCR | Microarray | qRT-PCR | Microarray |
| Up-regulated | ||||
| BCAR3 | 3.907 | 2.488 | 1.171 | 2.611 |
| CLIC4 | 31.636 | 21.46 | 24.750 | 11.710 |
| COL4A1 | 3.387 | 3.584 | 1.768 | 2.179 |
| COL4A2 | 2.967 | 7.692 | 1.834 | 2.725 |
| CXCR4 | 5.000 | 2.762 | 13.037 | 3.876 |
| KLF4 | 4.532 | 2.110 | 1.816 | 3.185 |
| LAMC2 | 1.633 | 3.003 | 2.276 | 3.676 |
| LAPTM5 | 22.135 | 24.331 | 37.060 | 5.917 |
| MAPKAPK3 | 3.665 | 3.861 | 7.056 | 3.831 |
| NR3C1 | 7.111 | 2.841 | 12.500 | 4.386 |
| PSAT1 | 1.394 | 2.278 | 2.876 | 6.897 |
| PTCH1 | 3.572 | 4.310 | 3.008 | 2.242 |
| RXRα | 10.953 | 5.405 | 5.750 | 2.445 |
| SLPI | 26.636 | 9.524 | 4.788 | 11.779 |
| Down-regulated | ||||
| ALDHIAI | 0.222 | 0.174 | 0.109 | 0.068 |
| AKR7A2 | 0.647 | 0.491 | 0.264 | 0.387 |
| C1orf186 | 0.366 | 0.155 | 0.278 | 0.265 |
| FZD5 | 0.365 | 0.440 | 0.552 | 0.380 |
| PAX2 | 0.145 | 0.247 | 0.041 | 0.382 |
| RAB25 | 0.012 | 0.076 | 0.002 | 0.082 |
aAll fold change values were statistically significant (P < 0.05); n = 2 replicates per cell line.