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Chicken Ψ-AMEL exon 2 analysis. Top: Alignment of chicken and crocodile AMEL exon 2 sequences. Four nucleotides are inserted (red) in chicken Ψ-AMEL exon 2 (signal peptide), leading to a shift in the reading frame. Middle: Putative deduced amino acid sequence from chicken Ψ-AMEL exon 2. Bottom: The four inserted codons were removed from the Ψ-AMEL sequence, which was translated and aligned to the crocodile sequence; both amino acid sequences are highly similar.
