Table 1.

The effect of p21 N and p21 C on in vivo DNA repair using permeabilized cells

Treatment	% Incorporation
Control (0 J/m2)	14.6  ± 12.4
UV (20 J/m2)	100
UV + p21 N
0.037 μM	97.7  ± 5.3
1.5 μM	101 ± 10.3
3.7 μM	103.8  ± 7.48
UV + p21 C
0.037 μM	92.4  ± 9.29
1.5 μM	75.1  ± 12.8
3.7 μM	52.0  ± 14.8

Normal human fibroblasts (GM38A) were encapsulated in 2.5% (Sigma type III) agarose at 39°C (see Materials and Methods). The encapsulated cells were irradiated with 254 nm UV radiation (20 J/m²). The cells were permeabilized in physiological buffer containing lysolecithin. p21 N and p21 C were added to the permeabilized encapsulated cells, and the reaction was incubated at 37°C with ATP and dNTPs. The samples were washed in ice-cold PBS and then lysed in 0.1% SDS. After lysis the samples were precipitated on filters with 5% TCA, washed, dried, and scintillation counted. Excluding p21 N at a concentration of 0.037 μM, all of the results are the mean of three independent biological experiments. Values are mean ± SD.