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. 2008 Oct 3;4(10):e1000171. doi: 10.1371/journal.ppat.1000171

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Overall neutralization activity against nine different HIV-1 Env pseudotyped viruses in serum samples from monkeys and rabbits following 4 immunizations with the YU2 gp140-F trimers. Sera were screened at a 1∶5 dilution (except against MW.965, which was done at a 1∶10 dilution of the sera) for neutralization activity. Neutralization between 70 and 79% is indicated in yellow, while neutralization between 80 and 100% is indicated in red. Pre-bleed serum sample effects on viral entry were negligible; BSA adjuvant control animals and MuLV pseudotyped virus were also included as negative controls. (B) Detection of CD4i antibodies in serum samples from immunized cynomolgus macaques and rabbits after 4 immunizations. Serum samples from monkeys and rabbits were titrated for neutralization activity against HIV-2_7312/V434M. Data are presented as the reciprocal dilution of immune serum resulting in a 50% inhibition of entry (ID₅₀) in the absence (white bars) or presence (blue bars) of 9 nM sCD4. (C) A 17b blocking assay was performed using sera elicited from monkeys (blue) and WT rabbits (black). For the assay, the sera were serially diluted in a 96 well format in which the wells were pre-coated with HXBc2 core gp120 glycoproteins. Following washing, biotinylated 17b was added to each well and the ability of the sera to block 17b binding was assessed by ELISA.