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. 2008 Oct 3;4(10):e1000168. doi: 10.1371/journal.ppat.1000168

Figure 5. In vivo phagocytosis in adult flies.

Figure 5

(A) Adult males of the indicated genotypes were injected with Alexa Fluor 488-labeled heat killed spores of B. bassiana. (B, C, E, G) Quantitation of in vivo phagocytosis of India ink, spore and bacteria. (B) India ink, (C) Alexa Fluor 488-labeled heat killed spores of B. bassiana, (E) Fluorescein conjugated E. coli (K-12), (G) Fluorescein conjugated S. aureus. Phagocytosed signals were observed under a Zeiss Axioplan 2 microscope. Phagocytic index was derived by multiplying the area of the India ink and fluorescence signal measured. Phagocytosis was inhibited by prior injection of latex beads in wild type. LXB, CML latex beads. (D, F, H) A phagocytic index was obtained by multiplying phagocytosing signals with the mean area of internalized India ink. (D) spore, (F) E. coli, (H) S. aureus. The mean and standard deviation of 10–16 adult flies were analyzed for each genotype. p-values were calculated by Student's t-test. India ink, **p<0.1. Fungi and bacteria, **p<0.007.