Figure 3. Loss of de2f2 does not affect the wts mutant phenotype in the eye.

Clones of mutant cells were generated with ey-FLP. Position of the morphogenetic furrow (MF) is marked by a white arrowhead in B and D. Posterior is to the right. (A) de2f2 and wts are on two separate chromosomal arms. Therefore following expression of ey-FLP, clones of cells of four different genotypes are generated (wild type, de2f2 mutant, wts mutant and de2f2 wts double mutant). de2f2 wts double mutant tissue is marked by the lack of GFP (green), is labeled e2f2^−/− wts^−/− in (A) and is outlined by a white line in (A–C). wts single mutant tissue is distinguished by a reduced intensity of GFP (green) and an increased spacing between ommatidial clusters (marked by ELAV). An example of wts mutant tissue is labeled wts^−/− in (A) and is denoted by yellow outline in (A–C). Wild type tissue is distinguished by the strongest level of GFP (green). An example of the wild type tissue is found between the yellow and white line. (B–D) Mosaic larval eye discs were labeled with BrdU (red) to detect cells in S phase (B,D) and ELAV (blue) to identify position of ommatidial clusters (B–D). Posterior to the MF, wild type cells undergo a single round of S phases in the second mitotic wave (SMW) (denoted by white arrow in B). In contrast, inappropriate BrdU (red) incorporation posterior to the SMW was detected in both wts and de2f2 wts mutant cells (B,D). (C) As a result of this inappropriate proliferation, spacing between ommatidial clusters is increased in both wts and de2f2 wts mutant tissue in comparison to wild type tissue. A merged image is shown in D.