Table 2.
Linearity, limit of detection (LoD) and limit of quantitation (LoQ) of the ESI-LC-MS/MS assay for hydroxy fatty acids, leukotrienes, resolvins and protectins. (LoD and LoQ are expressed as pg on-column).
| Compound | Equation | Correlation Coefficient |
LoD (pg) | LoQ (pg) |
|---|---|---|---|---|
| 9-HODE | y = 2.080x − 0.010 | 0.994 | 10 | 20 |
| 13-HODE | y = 0.483x − 0.028 | 0.987 | 20 | 50 |
| 5-HEPE | y = 0.511x + 0.021 | 0.998 | 20 | 50 |
| 18-HEPE | y = 0.279x + 0.010 | 0.994 | 20 | 50 |
| 9-HEPE | y = 0.321x − 0.016 | 0.996 | 20 | 50 |
| 8-HEPE | y = 0.877x + 0.037 | 0.972 | 20 | 50 |
| 15-HEPE | y = 0.459x + 0.016 | 0.999 | 20 | 50 |
| 12-HEPE | y = 0.484x − 0.030 | 0.999 | 20 | 50 |
| 5-HEPE | y = 0.564x + 0.050 | 0.986 | 20 | 50 |
| 9-HETE | y = 0.169x + 0.043 | 0.983 | 20 | 50 |
| 8-HETE | y = 2.688x + 0.073 | 0.997 | 10 | 20 |
| 11-HETE | y = 4.623x − 0.046 | 0.999 | 10 | 20 |
| 15-HETE | y = 1.083x − 0.013 | 0.998 | 10 | 20 |
| 12-HETE | y = 0.472x − 0.007 | 0.991 | 20 | 50 |
| LTB4 | y = 2.127x + 0.031 | 0.991 | 10 | 20 |
| 17S-HDHA | y = 1.404x + 0.044 | 0.992 | 10 | 20 |
| RvE1 | y = 1.949x + 0.208 | 0.972 | 20 | 50 |
| PD1 | y = 0.606x + 0.125 | 0.984 | 10 | 20 |
| RvD1 | y = 2.979x + 0.008 | 0.996 | 20 | 50 |