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. 2008 Jul 24;178(6):605–617. doi: 10.1164/rccm.200712-1822OC

Figure 10.

Figure 10.

Reductions in pre–B-cell colony enhancing factor (PBEF) availability is protective in ventilation-induced lung injury (VILI). (A) We initially assessed the effect of PBEF neutralizing antibody (PBEF Ab) on recombinant human PBEF (rhPBEF)–mediated neutrophil (PMN) influx into lung tissues and alveolar space (4.5 h, 20 μg/mouse). Simultaneous administration of rhPBEF and PBEF antibody (2 μg and 88 μg/mouse, ratio PBEF Ab/rhPBEF ratio of 0.1 and 4.4) resulted in marked reductions in PBEF-mediated accumulation of bronchoalveolar lavage (BAL) PMNs (*P < 0.05). (B, C) We next used PBEF neutralizing antibody (88 μg) via intratracheal delivery in VILIb-challenged B6 mice and determined that PBEF Ab reduces (B) VILI-induced PMN accumulation and (C) tissue albumin leakage (40 ml/kg) (n = 3–4), *P < 0.05. SB = spontaneously breathing mice.