Figure 1.

Representative gel images of RT-PCR analysis of total RNA using primers specific for GABA_B receptor subunits. (A) Splice variants (R1a/b/c [302 bp] and R1e [150 bp]) of GABA_BR1 were detected in human airway epithelium (Epi), human cultured bronchial epithelial cells (BEAS-2B), human lung epithelial adenocarcinoma cells (H441), guinea pig (GP) airway epithelium, and GP and human whole brain. (B) Messenger RNA encoding GABA_BR2 was detected in both human epithelial cell lines and in freshly isolated epithelium or whole brain from guinea pig and human. Images are representative of at least three independent RT-PCR analyses from each cell or tissue source.