Figure 4.

(A) Basal adenylyl cyclase activity in cultured BEAS-2B cells in the presence or absence of baclofen (100 μM) or SKF97541 (100 μM) (n = 6 experiments). Data represent means ± SEM. *P < 0.05, **P < 0.01 compared with control. (B) Forskolin (10 μM)-stimulated adenylyl cyclase activity in cultured BEAS-2B cells in the presence or absence of baclofen (100 μM) (n = 13). In some experiments, cells were pretreated with pertussis toxin (PTX) (100 ng/ml for 4 h pretreatment before baclofen treatment) (n = 10). Data represent means ± SEM. ***P < 0.001 compared with forskolin. In each experiment, values were determined in triplicate.