Figure 5.
(A) Immunoblot analyses of phospho-ERK or total ERK expression in cultured BEAS-2B cells. Cells were treated with baclofen (100 μM) (left) or SKF97541 (100 μM) (right) for 5 minutes in the presence or absence of PTX (100 ng/ml for 4 h pretreatment before GABAB selective agonist treatment). Representative immunoblots from five experiments are shown. (B) Relative band intensities of phospho-/ total ERK from five separate immunoblots. The effects of baclofen (left) and SKF97541 (right) on ERK phosphorylation are given as percentage of control. Data represent means ± SEM. *P < 0.05, **P < 0.01 compared with control. #P < 0.05, ##P < 0.01 compared with baclofen or SKF97541.