Figure 7.

Representative gel images of RT-PCR analysis of total RNA using primers specific for GAD65 and GAD67. (A) mRNA encoding GAD65 (488 bp) was detected in whole brain from both species and freshly isolated GP epithelium (Epi). (B) mRNA encoding GAD67 [375bp] was detected in whole brain from both species and in freshly isolated human epithelium and both human cultured epithelial cell lines (BEAS-2B and H441). Images are representative of at least three independent RT-PCR analyses from each cell or tissue source.