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Expression of S1P receptors in human lung fibroblasts. (A) mRNA expression. Total RNA was extracted from HFL-1 cells or Jurkat cells. RT-PCR was performed as described in Materials and Methods. (B) Immunoblots for S1P1, S1P2, and S1P5. Cell lysate from HFL-1 cells was subjected to immunoblotting for S1P1, S1P2, and S1P5. β-actin was used as a loading control.
